3D and 2D micro and nanostructures mimicking the biochemical and hierarchical organization of the natural extracellular matrix are fabricated by combining 3D/4D printing techniques with electrospinning and soft lithography. Hybrid systems composed of nanofillers and biodegradable polymers with the purpose to directing adhesion, proliferation, and differentiation of stem cells into specific lineages are additionally investigated to unveiling mechanisms at the base of the cell to cell and cell to materials interactions. The exploitation of physical and chemical stimuli is furthermore pursued to design novel implantable devices, intelligent system for regenerative nanomedicine, and for monitoring of physisological vital signs.
Scanning electron microscope images of scaffolds with different surface texturing. a) multilayered crosshatch mesh with line width of about 250 micrometers and b) nanofiber mesh with average fiber diameter of about 250 nanometers. c) lung epithelial cell cells adhering on crosshatch mesh.
| People | Andrea Camposeo, Jose Gustavo De La Ossa Guerra, Sujoy Ghosh, Luana Persano* |
| Keywords | Polymer/hybrid Nanoscaffolds and Nanofibers, 3D and 4D scaffolds, mechanotransduction |
| Methods, techniques | Electrospinning, soft lithographies, 3D printing |
| Granted projects | |
| PNRR Next Generation EU Tuscany Health Ecosystem – Spoke 4: Nanotechnologies for diagnosis and therapy | |
| Sinonasal cancer: In-depth genetic analysis of patients for personalized treatment and disease monitoring (ADAPTA), Regione Toscana, 2019-2022 | |
| Sujoy Kumar GHOSH, Bioresorbable Self-powered Implantable Device (BIOIMD), MSCA-IF-2019, 2020- 2022 | |
| Collaborations | University of Pisa, Azienda Ospedaliero Universitaria Pisana |
| Publications | |
| M E F. Palamà et al. Xeno-free cultured mesenchymal stromal cells release extracellular vesicles with a “therapeutic” miRNA cargo ameliorating cartilage inflammation in vitro, Theranostics 2023 | |
| M. f. L. Palamà et al. The Secretome Derived From Mesenchymal Stromal Cells Cultured in a Xeno-Free Medium Promotes Human Cartilage Recovery in vitro Front Bioeng Biotechnol 2020 | |
| A. Portone et al. Lineage-Specific Commitment of Stem Cells with Organic and Graphene Oxide–functionalized Nanofibers. Adv. Funct. Mater. 2019 | |
| A Portone et al. Quasi-3D morphology and modulation of focal adhesions of human adult stem cells through combinatorial concave elastomeric surfaces with varied stiffness Soft Matter 2019 |